Table 1.

Screening of allosteric effect of citalopram analogues on [³H]‐S‐CIT dissociation

Cpd (50 μM)	t 1/2 for [ 3 H ]S‐CIT dissociation (min)	n
Control	85 ± 6.9	10
S‐CIT	790 ± 160	4
2	380 ± 26	4
3	1090 ± 130	4
4	1170 ± 240	4
5	280 ± 70	4
6	260 ± 20	4
7	440 ± 30	4
8	570 ± 70	4
9	120 ± 10	4
10	110 ± 9.1	4
11	150 ± 18	4
12	150 ± 9.4	4
13	89 ± 6.5	4
14	85 ± 3.0	4
15	100 ± 15	4
Talopram	100 ± 3.8	4

Effect on t _1/2 (min) for [³H]‐S‐CIT dissociation by 50 μM of the indicated cpds. The t _1/2 values were calculated by non‐linear regression analysis of prebound [³H]‐S‐CIT dissociation on membrane preparations from COS7 cells transiently expressing SERT. Data are shown as mean ± SEM of n number of individual experiments. Due to the high temperature sensitivity of the assay, a control sample was always performed in parallel to the compounds, hence the high sample size for the control.