Fig. 5.

Effects of signal transducer and activator of transcription 1 (STAT1) andSTAT2 knockdown on interferon-induced transmembrane protein 1 (IFITM1) expression and tumorigenic potential of SUM149 cells. a Left panel: Western blots showing the protein levels of STAT1, STAT2, and IFITM1 after STAT1 and STAT2 genes were knocked down in SUM149 cells using siSTAT1 and siSTAT2. β-Actin was used as a loading control. Left panel: Quantification of the protein levels of IFITM1 in the siSTAT1, siSTAT2, and siSTAT1/2 samples. IFITM1 levels were quantified using ImageJ software, were normalized to β-actin, and were expressed relative to siControl. b Upper panel: Western blot of STAT2 knockdown in SUM149 cells and the effects of the knockdown on cell proliferation. Lower panel: Western blot of STAT1 knockdown in SUM149 cells and the effects of the knockdown on cell proliferation. c Left panel: Effects of STAT1 and STAT2 knockdown on 2-D colony formation in SUM149 cells. Right panel: Quantification of the colonies. d Wound-healing assay showing the effects of STAT1 and STAT2 knockdowns on the ability of SUM149 cells to migrate. *P < 0.05