Fig. 7.

Interferon-induced transmembrane protein 1 (IFITM1) promoter activity in SUM149 cells. a Schematic diagram of the IFITM1 promoter showing ATG transcription start site, interferon-stimulated response element (ISRE)/interferon regulatory factor (IRF), interferon gamma-activated site (GAS), TTA half-sites, and TTC half-palindromes; constructs; pGL3-IFITM1 − 750/−1, pGL3-IFITM1 − 200/−1, and the empty vector (pGL3). b Relative luciferase activity of the IFITM1 promoter region using the two deletion constructs (−750/−1 and −200/−1), along with interferon treatment in SUM149 cells. c Effects of signal transducer and activator of transcription 1 (STAT1) and STAT2 knockdown on the IFITM1 promoter activity using the −750/−1 deletion construct. The luciferase activity was expressed relative to scrambled control RNA (siCon). The data presented are representative of triplicate experiments and are expressed as mean ± standard deviation. *P < 0.05. RLU stands for Relative Light Units (RLU). It is a unit that is based on light emitted by the luciferase-catalyzed chemiluminescent reaction