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. 2016 Feb 20;18:25. doi: 10.1186/s13058-016-0683-7

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© Ogony et al. 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 8 — Activation of type I interferon (IFN) signaling pathway by exogenous IFNα in SUM149 and SUM190 cells. a Effects of IFN induction on the protein levels of IFN-stimulated genes (IFITM1, STAT1, p-STAT1, STAT2, and p-STAT2) in the SUM149 and SUM190 cells. β-Actin was used as a loading control. b Cellular localization and expression of interferon-induced transmembrane protein 1 (IFITM1) protein in SUM149 and SUM190 cells following IFN induction. Immunofluorescence was used to assess IFITM1 expression and localization in the cells that were induced with IFNα for 24 h as compared with the control. The images were captured using Leica confocal microscope. DAPI 4′,6-diamidino-2-phenylindole, STAT signal transducer and activator of transcription