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. 2016 Feb 19;47:33. doi: 10.1186/s13567-016-0318-0

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© Eck et al. 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Characterization of VRP-mediated expression of the GP3 ectodomain. A Immunofluorescence analysis of MARC-145 cells 6 h after infection with VSV*ΔG(GP3ecto-Myc) or with the VSV*ΔG control. Expression of GP3ecto is detected with a rabbit anti-Myc serum (top panels) and with the anti-GP3 mAb VII2D (bottom panels). B Western blot analysis of lysates (ly) and supernatants (sn) from cells infected with VSV*ΔG(GP3ecto-Myc) separated by SDS-PAGE under non-reducing (-βME) conditions, using the anti-Myc serum for GP3ecto-Myc detection (arrowhead). Protein molecular weight (kDa) standards are indicated.