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. 2016 Feb 19;47:33. doi: 10.1186/s13567-016-0318-0

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© Eck et al. 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Characterization of VRP-mediated expression of N. A Immunofluorescence analysis of MARC-145 cells 6 h after infection with VSV*ΔG(N) or with the VSV*ΔG control. The expression of N is detected with the anti-N mAb 13E2. B Western blot analysis of lysates from non-infected cells (lane 1) or cells infected with VSV*ΔG (lane 2), VSVΔ*G(N) (lane 3), PRRSV Olot/91 (lane 4) or mock (lane 5). The proteins were separated by SDS-PAGE under non-reducing (-βME) conditions and blotted onto a nitrocellulose membrane. N antigen was detected with the anti-N mAb as above. Protein molecular weight (kDa) standards are indicated.