Table 2.
Seroconversion after vaccination of pigs with VSVΔG(GP5/M) followed by PRRSV challenge.
| Ab against PRRSV Ag (by ELISA) | Vaccine | ELISA | Days after PRRSV challenge infection | |||||||
|---|---|---|---|---|---|---|---|---|---|---|
| 0 | 2 | 4 | 6 | 8 | 10 | 13 | 21 | |||
| GP5 | VSVΔG(GP5/M) | Positive/total pigs | 0/5 | 0/5 | 0/5 | 2/5 | 2/5 | 4/5 | 5/5 | 5/5 |
| % Competitiona (positive pigs) | 0 | 0 | 0 | 50 ± 12 | 49 ± 29 | 82 ± 15 | 82 ± 20 | 69 ± 15 | ||
| Range for positive pigs | 0 | 0 | 0 | 41–59 | 31–66 | 64–101 | 59–106 | 49–91 | ||
| VSV*ΔG (control) | Positive/total pigs | 0/5 | 0/5 | 0/5 | 0/5 | 0/5 | 0/5 | 0/5 | 2/5 | |
| % Competition (positive pigs) | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 62 ± 13 | ||
| Range for positive pigs | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 53–72 | ||
| N | VSVΔG(GP5/M) | Positive/total pigs | 0/5 | 0/5 | 0/5 | 0/5 | 0/5 | 2/5 | 4/5 | 4/5 |
| S/P ratiob (positive pigs) | 0 | 0 | 0 | 0 | 0 | 0.9 ± 0.4 | 1.0 ± 0.5 | 1.2 ± 0.1 | ||
| Range (positive pigs) | 0 | 0 | 0 | 0 | 0 | 0.5–1.2 | 0.6–1.7 | 1.1–1.3 | ||
| VSV*ΔG (control) | Positive/total pigs | 0/5 | 0/5 | 0/5 | 0/5 | 0/5 | 0(1)/5 | 4/5 | 3/5 | |
| S/P ratio (positive pigs) | 0 | 0 | 0 | 0 | 0 | 0 | 0.7 ± 0.2 | 1.4 ± 0.8 | ||
| Range (positive pigs) | 0 | 0 | 0 | 0 | 0 | 0 | 0.4–0.9 | 0.4–1.9 | ||
IDEXX X3 kit S/P ratios of >0.4 are considered positive. The mean values are indicated with the standard deviation.
a% Competition = (negative control mean [mean of optical absorbance] − sample mean)/(negative control mean − positive control mean) × 100. The mean values are indicated with the standard deviation.
bS/P ratio = (sample mean [mean of optical absorbance] − negative control mean)/(positive control mean − negative control mean).