Table 3.
Seroconversion after vaccination of pigs with VSVΔG(GP5/M) + VSVΔG(GP4/GP3/GP2) followed by PRRSV challenge.
| Ab against PRRSV Ag (by ELISA) | Vaccine | ELISA | Days after PRRSV challenge infection | |||||||
|---|---|---|---|---|---|---|---|---|---|---|
| 0 | 2 | 4 | 7 | 9 | 11 | 14 | 24 | |||
| GP5 | VSVΔG(GP5/M) + VSVΔG(GP4/GP3/GP2) | Positive/total pigs | 0/4 | 0/4 | 0/4 | 2/4 | 2/4 | 4/4 | 4/4 | 4/4 |
| % Competitiona (positive pigs) | 0 | 0 | 0 | 44 ± 0.6 | 48 ± 4 | 55 ± 10 | 89 ± 13 | 89 ± 11 | ||
| Range (positive pigs) | 0 | 0 | 0 | 43–44 | 45–51 | 45–65 | 72–102 | 77–100 | ||
| VSV*ΔG (control) | Positive/total pigs | 0/3 | 0/3 | 0/3 | 0/3 | 0/3 | 0/3 | 0/3 | 1/3 | |
| % Competition (positive pigs) | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 42 | ||
| Range (positive pigs) | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 42 | ||
| GP3 | VSVΔG(GP5/M) + VSVΔG(GP4/GP3/GP2) | Positive/total pigs | 0/4 | 0/4 | 0/4 | 3/4 | 4/4 | 4/4 | 4/4 | 4/4 |
| VSV*ΔG (control) | Positive/total pigs | 0/3 | 0/3 | 0/3 | 0/3 | 0/3 | 0/3 | 0/3 | 2/3 | |
| GP4 | VSVΔG(GP5/M) + VSVΔG(GP4/GP3/GP2) | Positive/total pigs | 0/4 | 0/4 | 0/4 | 0/4 | 0/4 | 0/4 | 1/4 | 2/4 |
| VSV*ΔG (control) | Positive/total pigs | 0/3 | 0/3 | 0/3 | 0/3 | 0/3 | 0/3 | 0/3 | 1/3 | |
| N | VSVΔG(GP5/M) + VSVΔG(GP4/GP3/GP2) | Positive/total pigs | 0/4 | 0/4 | 0/4 | 0/4 | 0/4 | 0/4 | 1/4 | 4/4 |
| S/P ratiob (positive pigs) | 0 | 0 | 0 | 0 | 0 | 0 | 0.5 | 1.4 ± 0.4 | ||
| Range (positive pigs) | 0 | 0 | 0 | 0 | 0 | 0 | 0.5 | 0.9–1.9 | ||
| VSV*ΔG (control) | Positive/total pigs | 0/3 | 0/3 | 0/3 | 0/3 | 1/3 | 1/3 | 1(1)/3 | 3/3 | |
| S/P ratio (positive pigs) | 0 | 0 | 0 | 0 | 0.5 | 0.7 | 0.8 | 1.4 ± 0.7 | ||
| Range (positive pigs) | 0 | 0 | 0 | 0 | 0.5 | 0.7 | 0.8 | 0.8–2.1 | ||
a% Competition = (negative control mean [mean of optical absorbance] − sample mean)/(negative control mean − positive control mean) × 100. The mean values are indicated with the standard deviation.
bS/P ratio = (sample mean [mean of optical absorbance] − negative control mean)/(positive control mean − negative control mean). IDEXX X3 kit S/P ratios of >0.4 are considered positive. The mean values are indicated with the standard deviation.