Table 4.
Seroconversion after vaccination of pigs with VSV*ΔG(N), VSV*ΔG(GP3ecto-Myc), VSV*ΔG(GP4-HA) and VSV*ΔG expressing IAV proteins.
| Ab against PRRSV Ag (by ELISA) | Vaccine | ELISA | Days after VRP vaccination | Days after PRRSV challenge | ||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 0 | 14 | 28 | 63a | 77 | 0 | 2 | 4 | 7 | 9 | 11 | 14 | 21 | 28 | |||
| N | VSV*ΔG(N) | Positive/total pigs | 0/2 | n.d. | 0/2 | 2/2 | 2/2 | n.d. | n.d. | n.d. | 2/2 | n.d. | n.d. | 2/2 | n.d. | n.d. |
| S/P ratiob (positive pigs) | 0 | n.d. | 0 | 0.7 | 0.9 | n.d. | n.d. | n.d. | 2.3 | n.d. | n.d. | 3.0 | n.d. | n.d. | ||
| Range (positive pigs) | 0 | n.d. | 0 | 0.6–0.7 | 0.8–1.0 | n.d. | n.d. | n.d. | 2.0–2.6 | n.d. | n.d. | 2.9–3.0 | n.d. | n.d. | ||
| VSV*ΔG (control) | Positive/total pigs | 0/2 | n.d. | n.d. | n.d. | 0/2 | 0/2 | 0/2 | 0/2 | 0/2 | 0/2 | 0/2 | 1/2 | 2/2 | 2/2 | |
| S/P ratio (positive pigs) | 0 | n.d. | n.d. | n.d. | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.6 | 0.7 | 0.7 | ||
| Range (positive pigs) | 0 | n.d. | n.d. | n.d. | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.6 | 0.6–0.7 | 0.7–0.8 | ||
| GP3 | VSV*ΔG(GP3ecto-Myc) | Positive/total pigs | 0/2 | n.d. | n.d. | n.d. | 0/2 | n.d. | n.d. | n.d. | 0/2 | n.d | n.d. | 2/2 | 2/2 | n.d. |
| VSV*ΔG (control) | Positive/total pigs | 0/2 | n.d. | n.d. | n.d. | 0/2 | n.d. | n.d. | n.d. | 0/2 | n.d. | n.d. | 0/2 | 0/2 | n.d. | |
| N | VSV*ΔG(GP3ecto-Myc) | Positive/total pigs | 0/2 | n.d. | n.d. | n.d. | n.d. | 0/2 | 0/2 | 0/2 | 0/2 | 0/2 | 0/2 | 1/2 | 1/2 | 2/2 |
| S/P ratio (positive pigs) | 0 | n.d. | n.d. | n.d. | n.d. | 0 | 0 | 0 | 0 | 0 | 0 | 0.5 | 1.3 | 1.0 | ||
| Range (positive pigs) | 0 | n.d. | n.d. | n.d. | n.d. | 0 | 0 | 0 | 0 | 0 | 0 | 0.5 | 1.3 | 0.5–1.6 | ||
| GP4 | VSV*ΔG(GP4-HA) | Positive/total pigs | 0/2 | n.d. | n.d. | n.d. | 0/2 | n.d. | n.d. | n.d. | 0/2 | n.d. | n.d. | 2/2 | 2/2 | n.d. |
| VSV*ΔG (control) | Positive/total pigs | 0/2 | n.d. | n.d. | n.d. | 0/2 | n.d. | n.d. | n.d. | 0/2 | n.d. | n.d. | 0/2 | 0/2 | n.d. | |
| N | VSV*ΔG(GP4-HA) | Positive/total pigs | 0/2 | n.d. | n.d. | n.d. | n.d. | 0/2 | 0/2 | 0/2 | 0/2 | 0/2 | 0/2 | 1(1)/2 | 2/2 | 2/2 |
| S/P ratio (positive pigs) | 0 | n.d. | n.d. | n.d. | n.d. | 0 | 0 | 0 | 0 | 0 | 0 | 0.6 | 0.6 | 0.7 | ||
| Range (positive pigs) | 0 | n.d. | n.d. | n.d. | n.d. | 0 | 0 | 0 | 0 | 0 | 0 | 0.6 | 0.6–0.7 | 0.7 | ||
| HA | VSV*ΔG(HABelz) | Positive/total pigs | 0/2 | 2/2 | 2/2 | 2/2 | 2/2 | n.a. | n.a. | n.a. | n.a. | n.a. | n.a. | n.a. | n.a. | n.a. |
| NA | VSV*ΔG(NABelz) | Positive/total pigs | 0/2 | 2/2 | 2/2 | 2/2 | 2/2 | n.a. | n.a. | n.a. | n.a. | n.a. | n.a. | n.a. | n.a. | n.a. |
| NP | VSV*ΔG(NPBelz) | Positive/total pigs | 0/2 | 2/2 | 2/2 | 2/2 | 2/2 | n.a. | n.a. | n.a. | n.a. | n.a. | n.a. | n.a. | n.a. | n.a. |
| M2 | VSV*ΔG(M2Belz) | Positive/total pigs | 0/2 | 2/2 | 2/2 | 2/2 | 2/2 | n.a. | n.a. | n.a. | n.a. | n.a. | n.a. | n.a. | n.a. | n.a. |
n.d.: not done; n.a.: not applicable.
aAntibodies against GP3 and GP4 were also analysed at day 56. The results were 0/2.
bS/P ratio = (sample mean [mean of optical absorbance] − negative control mean)/(positive control mean − negative control mean). IDEXX X3 kit S/P ratios of >0.4 are considered positive. The S/P ratios represent the mean of 2 pigs, with the absolute values indicated under “range”.