Fig. 7.

Heatmap–cellular stress response: this heatmap demonstrates the change in expression patterns of genes involved in cellular stress response and homeostasis that displayed differential expression in at least one acclimation time point (7d, 28d, or 56d) when fish acclimated to the multi-stressor treatment (n = 4) were compared to fish in the control treatments (n = 4). Genes associated with the gene ontology term response to stress (GO:0006950) are represented by (x), immune system process (GO:0002376) are represented by (+), and homeostatic process (GO:0042592) are represented by (=) that displayed differential expression (FDR ≤ 0.05) in at least one time point are included. Fold change is log₂ adjusted with genes showing significant up-regulation depicted in yellow and genes showing a significant down-regulation in blue. Genes whose level of expression did not differ significantly between control and multi-stressor treatments are shown in white. The number of gene variants identified in the annotated transcriptome that were associated with the same gene name are indicated in parentheses next the gene symbol