Fig. 1.

Wildtype p21Ras proteins and anti-p21Ras monoclonal antibodies. a The plasmid map of recombinant pET-28a(+) vector expressing H-ras, in which H-ras gene CDS with Bam HI and Hind III restriction enzyme sites was cloned into a pET-28a(+) vector with a histidine tag-compatible end. b The three recombinant pET28a(+) plasmids were digested into two fragments by Bam HI and Hind III, the 570 bp of the ras gene CDS and 5340 bp of the pET-28a(+) vector. c SDS–PAGE analysis showed that the molecular weight of the three p21Ras proteins with His tag was 25 kDa. d KGH-R1 monoclonal antibody against three p21Ras proteins demonstrated strong immunoreactivity to cancer tissues but negative immunoreactivity to corresponding normal tissues