Fig. 7.

Immunolocalisation of Yorkie and EdU labelling of proliferative nuclei in the C. hemisphaerica exumbrellar epidermis. a Partial view of Dapi-stained medusa; focus on the exumbrellar epidermis. b EdU staining (following 2 h pulse) in the same medusa. cir can circular endodermal canal; tt bu tentacle bulb; z cen central region of the exumbrellar epidermis; z per peripheral region of the exumbrellar epidermis. The asterisk in b corresponds to an artefact. c–e Detailed view of the central area of the exumbrellar epidermis (z cen in a, b; blue in Fig. 8a) with anti-Yki (red in c, e), EdU (green in c, e) and Dapi (white in d; blue in e) staining. f–i Higher magnification view of the area boxed with dotted line in (e), showing Dapi (f), EdU (g) and anti-Yki (h) staining; i combination of the three signals (anti-Yki in red, Dapi in blue and EdU in green). j–l Detailed view of the peripheral area of the exumbrellar epidermis (z per in a, b; red in Fig. 8b) with anti-Yki (red in j, l), EdU (green in j, l) and Dapi (white in k; blue in l) staining. m–p Higher magnification view of the area boxed with dotted line in (l), showing Dapi (m), EdU (n) and anti-Yki (o) staining; (p) combination of the three signals (anti-Yki in red, Dapi in blue and EdU in green). q Graph of fluorescence intensities for Dapi, EdU and anti-Yki (see colour legend on the right), showing their quantified variation based on the picture in i along the yellow arrow (distance in abscisse is from the proximal extremity of the arrow). r Graph of fluorescence intensity for Dapi, EdU and anti-Yki (see colour legend on the left), showing their quantified variation based on the picture in p along the yellow arrow (distance in abscisse is from the proximal extremity of the arrow). Scale bars a, b 50 µm, c, h 20 µm, i, p 10 µm