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. Author manuscript; available in PMC: 2016 Feb 20.
Published in final edited form as: J Am Chem Soc. 2011 Mar 23;133(14):5178–5181. doi: 10.1021/ja111173c

Figure 3.

Figure 3

Addition of MAGE to differentiating 3T3-L1 cells. a) Supplementation of DMI with C18:1 MAGE (20 μM) from day 0 to 4 in 3T3-L1 cells led to increased triglyceride accumulation and number of adipocytes, as assessed by Oil Red O staining of neutral lipid content 8 days post-induction. b) C18:1 MAGE was added to the DMI induction medium at a range of 2–20 μM for days 0 to 4. Cells were fixed and stained on day 7, and the absorbance of extracted Oil Red O stain was monitored at 510 nm (n = 8; *, p < 0.01, Student’s t test). c) 1-Oleoylglycerol (MAG) or 1-(octadec-1-enyl)glycerol (MAGVE) was added to the DMI induction medium at 10 μM for days 0 to 2. Cells were fixed and stained on day 8, and absorbance of extracted Oil Red O stain was measured (n = 3; *, p < 0.01).

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