Skip to main content
. Author manuscript; available in PMC: 2016 Feb 20.
Published in final edited form as: J Am Chem Soc. 2011 Mar 23;133(14):5178–5181. doi: 10.1021/ja111173c

Figure 4.

Figure 4

Effect on gene expression of MAGE addition for different time periods. a) C18:1 MAGE (20 μM) was added to differentiating 3T3-L1 cells during different periods of the DMI treatment: days 0–2, 2–4, and 0–4. b) Relative expression levels of PPARγ target genes, adiponectin and aP2, assessed by RT-PCR using 36B4 as the reference gene. Relative mRNA levels were calculated with respect to the day 0 control using the comparative Ct method. Cells were harvested 96h after induction with DMI supplemented with C18:1 MAGE for the indicated times (n = 3; *, p < 0.01).

HHS Vulnerability Disclosure