Figure 9. Chromosomal Rearrangements.

A. Generation of a deletion using recombination sites located in cis. B. Generation of a reciprocal deletion and duplication using recombination sites located in trans. C. Generation of an inversion using recombination sites located in cis. Such inversions can be similarly generated using shared homology instead. D. Generation of an engineered balancer chromosome using recombination sites located in cis. Reconstitution of 5’- and 3’-parts of a marker results in visualization of the inversion event. E. Generation of a translocation between two non-homologous chromosomes using recombination sites located in trans. Such translocation can be similarly generated using shared homology instead. F. Generation of a translocation between two homologous chromosomes using recombination sites located in trans during mitotic recombination. This experimental paradigm can be further developed to include strategies for clonal marking, i.e., here the Mosaic Analysis with a Repressible Cell Marker (MARCM) is illustrated (Lee and Luo, 1999).