Table 1.
Recombinase | Target Site | Sequencea |
---|---|---|
Cre | LoxP | 5’-ATAACTTCGTATAatgtatgcTATACGAAGTTAT-3’ |
Lox2272 | 5’-ATAACTTCGTATAaagtatccTATACGAAGTTAT-3’ | |
Loxm2 | 5’-ATAACTTCGTATAagaaaccaTATACGAAGTTAT-3’ | |
LoxLE | 5’-TACCGTTCGTATAatgtatgcTATACGAAGTTAT-3’ | |
LoxRE | 5’-ATAACTTCGTATAatgtatgcTATACGAACGGTA-3’ | |
Fre | LoxH | 5’-ATATATACGTATAtatgtctaTATACGTATATAT-3’ |
Tre | LoxLTR | 5’-ACAACATCCTATTacaccctaTATGCCAACATGG-3’ |
FLP | FRT | 5’-GAAGTTCCTATTCtctagaaaGTATAGGAACTTC-3’ |
F3 | 5’-GAAGTTCCTATTCttcaaataGTATAGGAACTTC-3’ | |
F5 | 5’-GAAGTTCCTATTCttcaaaagGTATAGGAACTTC-3’ | |
FRT-LE | 5’-GAAGTTCATATTCtctagaaaGTATAGGAACTTC-3’ | |
FRT-RE | 5’-GAAGTTCCTATTCtctagaaaGTATATGAACTTC-3’ | |
mFlp5 | mFRT71 | 5’-GAAGTTTCTATTCtctagaaaGTATAGAAACTTC-3’ |
Dre | Rox | 5’-TAACTTTAAATAATgccaATTATTTAAAGTTA-3’ |
KD | KDRT | 5’-AAACGATATCAGACATTTGTCTGATAATgcttcATTATCAGACAAATGTCTGATATCGTTT-3’ |
B2 | B2RT | 5’-GAGTTTCATTAAGGAATaactaATTCCCTAATGAAACTC-3’ |
B3 | B3RT | 5’-GGTTGCTTAAGAATaagtaATTCTTAAGCAACC-3’ |
R | RSRT | 5’-TTGATGAAAGAATAacgTATTCTTTCATCAA-3’ |
VCre | VloxP | 5’-TCAATTTCTGAGAactgtcatTCTCGGAAATTGA-3’ |
SCre | SloxP | 5’-CTCGTGTCCGATAactgtaatTATCGGACATGAT-3’ |
Vika | Vox | 5’-AATAGGTCTGAGAacgcccatTCTCAGACGTATT-3’ |
Inverted repeats are in capital, spacers are in lower case, mutations compared to wild type site are highlighted in red