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. Author manuscript; available in PMC: 2017 Mar 1.
Published in final edited form as: Exp Neurol. 2016 Jan 16;277:305–316. doi: 10.1016/j.expneurol.2016.01.011

Figure 2. PAC expression in Chx10-Puro ESC line allows for enrichment of neuronal cells.

Figure 2

(A) Chx10 and PAC mRNA expression in ESCs, ESCs induced with RA and puromycin (Pur), and ESCs induced with RA and cyclopamine (Cyc). (B) Schematic of 2−/4+induction protocol followed by 24 hours of puromycin (Puro) selection. (C) Live cell viability assay (Scale bars = 100 μm) and (D) flow cytometry in ESCs, ESCs induced with RA and Pur, and ESCs induced with RA and Cyc following puromycin selection with 2 μg/mL or 4 μg/mL; control cultures without puromycin were run in parallel. *p<0.0001 compared to control group. (E) Phase contrast with tdTomato overlay demonstrating neuronal morphology in selected cultures (Scale bar = 50 μm).