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. Author manuscript; available in PMC: 2017 Mar 1.
Published in final edited form as: Exp Neurol. 2016 Jan 16;277:305–316. doi: 10.1016/j.expneurol.2016.01.011

Figure 6. Currents activated by transmitter receptor agonists in selected Chx10-Puro cells.

Figure 6

(A) Whole-cell currents evoked by brief application of 100 μM kainate, GABA, glycine or NMDA (plus 1 μM glycine) as indicated by the filled bars. Holding potential, −80 mV; 9 days after puromycin selection (* denotes significant difference from d3 and d9, # denotes significant difference from d3). (B) Peak agonist-gated current at −80 mV (mean ± SEM) recorded in cells 3, 9 or more than 20 days after puromycin selection. (C) Agonist-evoked currents recorded during voltage ramps from −110 to +110 mV at 1.2 mV/msec, 28 days after puromycin selection. GABA and glycine evoked currents that reversed polarity at −53.8 ± 1.7 mV (n=4) and −55.3 ± 1.7 mV (n=4), respectively, consistent with activation of chloride-selective channels. Currents evoked by kainate and NMDA reversed polarity at 5.9 ± 2.6 mV (n=4) and 19.8 ± 4.1 mV (n=4), respectively, consistent with monovalent cation permeability.