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. Author manuscript; available in PMC: 2017 Mar 1.
Published in final edited form as: Exp Neurol. 2016 Jan 16;277:305–316. doi: 10.1016/j.expneurol.2016.01.011

Figure 8. Evoked excitatory synaptic transmission between selected Chx10-Puro cells.

Figure 8

Simultaneous whole-cell recordings of pre-and postsynaptic currents from two adjacent Chx10-Puro cells 31 days after puromycin selection. Voltage steps to 0 mV from a holding potential of −80 mV elicited inward sodium current, and uncompensated capacity transients, in the presynaptic cell. Excitatory synaptic currents (EPSCs) were evoked in the postsynaptic cell with a delay of 3.2 msec, measured from the peak of presynaptic sodium current to the initial rise of the EPSC. Exposure to the AMPA/kainate receptor antagonist NBQX (30 μM) completely blocked the EPSC, while the GABAA receptor antagonist bicuculline methiodide (0.2 mM) had no effect. Each panel shows 3 superimposed traces in each condition. Presynaptic stimuli were delivered at 0.2 Hz; postsynaptic holding potential, −80 mV.