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. Author manuscript; available in PMC: 2017 Feb 1.
Published in final edited form as: Virology. 2016 Jan 14;489:282–291. doi: 10.1016/j.virol.2015.12.015

Fig 2.

Fig 2

CypB expression in primary PBMCs. (A) Infection of donor-derived lymphocytes. Cells were activated with PHA and infected with NLX virus. Virus replication was monitored by RT assay of cell supernatants using an in vitro [32P]dTTP incorporation assay. Results presented are representative of triplicate experiments. (B) Unstimulated, PHA-stimulated, and PHA-stimulated infected PBMCs were fractionated into cytosolic (Cyt), membrane/organelle (Mem), nuclear (Nu), and cytoskeletal/insoluble (In) fractions. Equivalent amounts of each fraction was separated by SDS-PAGE and immunoblotted for CypB. Example blots of the controls used for fraction integrity and protein concentration are shown in bottom three panels.