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. Author manuscript; available in PMC: 2017 Feb 1.
Published in final edited form as: Virology. 2015 Dec 18;489:51–62. doi: 10.1016/j.virol.2015.12.001

Figure 1.

Figure 1

Nucleolin is expressed in the surface of CrFK cells. A) Non-permeabilized and permeabilized CrFK cells with acetone, were stained with an anti-NCL antibody (green), and visualized by indirect immunofluorescence using Alexa 488 as secondary antibody. DAPI was used for nuclei staining (blue). B) Non-permeabilized CrFK cells were incubated with anti-NCL (black line) or anti-rabbit IgG antibodies (gray shaded area), used as an isotype control. C) CrFK cells were treated with sulfosuccinimidyl-6-(biotin-amide) hexanoate (+) or vehicle (−) and biotinylated proteins were precipitated with streptavidin-agarose, and separated by SDS-PAGE. NCL and hnRNP A1 proteins were detected by immunoblotting using specific anti-NCL and anti-hnRNP A1 antibodies respectively.