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. Author manuscript; available in PMC: 2017 Feb 1.
Published in final edited form as: Virology. 2015 Dec 18;489:51–62. doi: 10.1016/j.virol.2015.12.001

Figure 3.

Figure 3

Anti-NCL antibody an FL NCL soluble protein does not have an effect on FCV infection in CrFK cells. A) Non treated and CrFK cells treated for 1 h at 37°C with anti-NCL, anti-rabbit IgG, anti-JAM-1 antibodies, and JAM-1 preimmune serum, were non infected or infected with FCV at an MOI of 0.01 for 24h. Cytopathic effect was observed by phase contrast microscopy. B) The bar chart illustrates the virus yield in supernatants determined by plaque assay. Error bars represent the standard deviations from three independent experiments. Value of P ≤ 0.05 (*) is indicated. C) Pre-incubated FCV (0.01 MOI) with BSA or FL rNCL proteins were added or not (no treatment) to CrFK cells for 24 h, and virus yield was determined in the supernatants by plaque assay. D) CrFK cells treated for 45 min on ice with AGRO-100 (gray shaded area) or a mix of 1μM FL rNCL (dashed line) or rGST (black line) proteins pre-incubated with 5μM FITC-AGRO for 2 h at 4°C. Mean fluorescence intensity (MFI) of FITC-AGRO100 was analyzed by flow cytometry.