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. Author manuscript; available in PMC: 2017 Feb 1.
Published in final edited form as: Virology. 2015 Dec 18;489:51–62. doi: 10.1016/j.virol.2015.12.001

Figure 5.

Figure 5

AGRO100 inhibits FCV in vitro translation. A) Total extracts from CrFK cells, ΔrNCL and extracts from RRL (lanes 1, 2 and 3 respectively) were analyzed by SDS-PAGE and NCL was detected by western blot using a specific monoclonal antibody. B) FCV in vitro translation was performed in RRL extracts and the 35S methionine labeled viral proteins were analyzed by SDS-PAGE in the absence (lane 2) and the presence of increasing concentrations of AGRO100 (lanes 3–7), or oligo dT (lane 8) used as a non-specific control. C) The bar chart illustrates the amount of FCV protein synthesis as percentage respect of the control (0 μg AGRO100) as measured by ImageJ quantification. Error bars represent the standard deviation from three different experiments.