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. 2015 Nov 13;33:151–165. doi: 10.1007/s10585-015-9765-7

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© The Author(s) 2015

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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Fig. 4 — Endo180 is upregulated in metastatic prostate cancer cells cultured on human stromal cell-derived ECM surfaces. a Flow cytometry analysis of PC3, VCaP and DU145 cells using three anti-human Endo180 monoclonal antibodies (A5/158, E1/183, 39.10). b Immunofluorescent staining of Endo180 (A5/158 mAb) in PC3-Endo180 cells cultured on plastic and ECM generated by primary human trabecular bone osteoblasts (osteoblast ECM); scale bar = 50 μm. c Relative levels of Endo180 expression (integrated fluorescent intensity; A5/158 mAb immunostaining) in PC3, PC3-Endo180 and DU145 cells cultured on tissue culture plastic (plastic) and ECM generated by primary human trabecular bone osteoblasts (osteoblast ECM) and human HCA2 dermal fibroblasts (fibroblast ECM); average ± s.d. values for three individual experiments, within which each experimental condition was tested in quadruplicate, are shown; significant differences compared to plastic are indicated (*p < 0.05). d Immunoblot shows Endo180 expression (A5/158 mAb) in VCaP cells cultured on plastic, rat-tail type I collagen (collagen), osteoblast ECM and fibroblast ECM for 6, 24 and 48 h (GAPDH = loading control). e Relative levels of Endo180 expression in (d) quantified using densitometry (n = 1)