Figure 2. Survival of mature B cells is survivin-independent.

(A) Flow cytometric analysis of splenocytes from survivin^L/Lcd21^Cre and survivin^+/+cd21^Cre mice with indicated antibodies. (B) Enumeration of splenocytes and splenic B cells in survivin^L/Lcd21^Cre and survivin^+/+cd21^Cre mice. (C) Immunohistology of spleens from survivin^L/Lcd21^Cre and survivin^L/+cd21^Cre mice. Red = B220+ B cells; Blue = CD3+ T cells; Green = moma-1+ macrophages. (D) Survivin^L/Lcd21^Cre and survivin^+/+cd21^Cre mice were continuously provided BrdU in the drinking water for a 7 week period. The graphs show the percentage of BrdU-positive cells in the indicated B cell subpopulations in the spleen and BM. (E) Deletion efficiency of survivin in LPS (10 µg/mL) stimulated B cells from survivin^L/Lcd21^Cre and survivin^+/+cd21^Cre mice after 2 days of culture.