Figure 4. Survivin is required for TI-2 and TD antibody responses.

(A) Relative concentration of TNP-specific IgM in sera from survivin^L/Lcd21^Cre and survivin^+/+cd21^Cre mice immunized with TNP-Ficoll. (B) Relative concentration of SRBC specific IgM and IgG1 in the sera from survivin^L/Lcd21^Cre and survivin^+/+cd21^Cre mice immunized with SRBC. (C) Relative frequency of GC B cells (B220⁺, GL7⁺, Fas⁺) within the splenic B cell compartment of survivin^L/Lcd21^Cre and survivin^+/+cd21^Cre mice 7 days post-SRBC immunization. (D) Immunohistology of spleens from survivin^L/Lcd21^Cre and survivin^+/+cd21^Cre mice 7 days post-SRBC immunization. B220 was used to detect B cells. PNA was used to detect GC B cells and CD35 was used to detect follicular dendritic cells. (E) Percentage of IgG1+ GC B cells on day 7 post-SRBC immunization. (F) Frequency of GC B cells within the B cell compartment in Peyer‘s Patches isolated from unimmunized survivin^L/Lcd21^Cre and survivin^+/+cd21^Cre mice. (G) Expression levels of phospho-H2AX in GC B cells from Peyer‘s patches isolated from unimmunized survivin^L/Lcd21^Cre and survivin^+/+cd21^Cre mice. Histograms are representative of 3 independent experiments.