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. Author manuscript; available in PMC: 2017 Mar 1.
Published in final edited form as: Cell Tissue Res. 2015 Sep 4;363(3):791–803. doi: 10.1007/s00441-015-2273-x

Fig. 3. Configurations of S3 segment proximal tubular cells.

Fig. 3

a, b: Two-micrometer paraffin sections, NBT-perfused and Neutral Red counterstained. In both micrographs, diformazan-stained cells are found in groups, and in some cases (arrows) doublets of nuclei are closely apposed.

c: Detail of cross-sectioned pars recta tubule. The diformazan-positive profile contains two nuclear profiles.

d: Semithin (<0.25 μm) plastic section, glutaraldehyde-fixed, unosmicated tissue with basic fuchsin counterstaining. A characteristic cell is identifiable by the deposition of diformazan particles (cf. Fig. 2 i-k), and a doublet of nuclei, similar to that seen in c, is visible within its confines (arrows).

Scale bar in b = 25 μm and applies to a and b; scale bar in d = 10 μm and applies to c and d.