TABLE 1.

Staining of Individual Mitochondrion-Rich S3 Segment Proximal Tubular Cells in Adult Mice

Substance	Source	Product No.	Host/MC/PC	Dilution	Positive or Negative
CD-44	Abcam	ab-41478	Rb PC	1:100	+
Cyclin D1	Thermo Scientific	RC-010	Rb PC	1:200	+
E-cadherin	Santa Cruz	sc-7870	Rb PC	1:250	+
Ferroportin	Santa Cruz	sc-49668	Gt PC	1:200	+
Fibronectin	Abcam	ab-6328	Ms MC	1:200	+
4-hydroxy-nonenal	Abcam	ab-48506	Ms MC	1:1000	+
iNOS	Santa Cruz	sc-7271	Ms MC	1:250	+
Ki-67	Abcam	ab-66195	Rb PC	1:250	+
Lotus tetragonolobus agglutinin	Vector Laboratories				± apical cytoplasmic staining
LSD-1	Abcam	ab-17721	Rb PC	1:1500	+
Megalin/Lrp2	Abcam	ab-76969	Rb PC	1:500	± apical cytoplasmic staining
NBT (nitroblue tetrazolium)	Sigma-Aldrich	N5514			+
Notch1	Santa Cruz	sc-9170	Rb PC	1:100	+
Notch2	Santa Cruz	sc-5545	Rb PC	1:100	+
Nitrotyrosine	Millipore	06-284	Rb PC	1:1000	+
Osmication					+
PAS	Electron Microscopy Sciences				Not specific: intrinsically darker cytoplasts
PAX-2	Zymed Labs.	71-6000	Rb PC	1:200	+
Renin	Gift from Dr. Tadashi Inagami Vanderbilt U.		Gt PC	1:10,000	+
Silver methenamine (Jones silver stain)					Nuclei highly argentophilic
α-SMA	Sigma-Aldrich	A-2547	Ms MC	1:800	-
Transgelin	Gift from Dr. ShuMan Fu University of Virginia		Rb PC	1:1500	-
Trichrome	Sigma-Aldrich				+ (again on basis of overall darker cytoplasm)
TUNEL ApopTag®	Millipore	S-7100			-
Vimentin	Abcam	ab-45939	Rb PC	1:2500	+

Abcam/Cambridge, MA

Electron Microscopy Sciences/Hatfield PA

Millipore/Temecula, CA

Polysciences/Warrington, PA

Santa Cruz Biotechnology/Santa Cruz, CA

Sigma-Aldrich/St. Louis, MO

Thermo Scientific/ Suwannee GA

Vector Laboratories/Burlingame, CA

Zymed Laboratories/San Francisco CA