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. Author manuscript; available in PMC: 2017 Mar 1.
Published in final edited form as: J Immunol. 2016 Jan 29;196(5):2004–2009. doi: 10.4049/jimmunol.1501557

FIGURE 3.

FIGURE 3

In vivo viral infection (LAIV) increases glycolysis in human pDCs and correlates with pDC antiviral responses. Blood pDCs were purified from healthy donors before (D0), 1 (D1) and/or 3 (D3) days after LAIV and pDCs (5×104) were cultured ex vivo for 24 h. (A) 13C-lactate quantification and (B) lactate efflux rate in pDC supernatants. (C) Relative pDC mRNA expression of HK2 and LDHA. (D) IFN-α2a secretion (left) and relative IRF7 expression (right) in pDCs cultured ex vivo for 24 h. (E) Effect of LAIV on pDC HLA-DR expression. (F) Nasal and T lymphocyte responses to LAIV. Nasal cell IRF7 expression normalized to PPIA (left); Flu-specific IFN-γ secreting T cells measured by ELISPOT before (D0) and after (day 8–10) LAIV (right). (G) Correlations between pDC glycolysis (lactate production) and HLA-DR expression (upper) and IFN-α secretion (lower). Data from 3 time points (D0, D1 and D3) displayed. Data in histograms represent mean ± SEM. In A and E, lines connect data from individual donors. N= 6–11 *p ≤ 0.05, paired t-test.