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. Author manuscript; available in PMC: 2017 Mar 1.
Published in final edited form as: J Immunol. 2016 Jan 25;196(5):2300–2308. doi: 10.4049/jimmunol.1500130

Figure 6. Super-low dose LPS promotes neutrophil migration via the p38 MAPKs pathway.

Figure 6

(A) Chemotaxis of neutrophils pretreated with 20 μM SB203580 (p38 inhibitor) or 20 μM SP600125 (JNK inhibitor) (2 h prior to the assay) against chemokine MIP-2. Representative images were shown. (B) Quantification of neutrophils that migrated into the gels toward MIP-2. (C) Phosphorylation of p38 and JNK MAPKs and expression of MKP7 in neutrophils treated with or without LPS for 24 h. Images are representative of, and protein expression profiles are pooled from, 3 independent experiments. *P < 0.05, **P < 0.01 versus the control group. (D) In vivo activated memory state of neutrophils. HFD-fed ApoE−/− mice injected were injected with either PBS or super-low dose LPS twice-weekly for one month as described in the Materials and Methods. Following the stoppage of injection, mice were continually fed with HFD for an additional month. Peripheral blood leukocytes and bone marrow cells were stained with fluorescent antibodies, and the expression levels of CD14 on CD11b+Ly6G+ neutrophils gated within the Ly6C− population were examined by flow cytometry. Error bars show means ± s.e.m.;** P <0.01; student t-test.