CpG-1826 elicits tumor growth delay and immune activation in GL261 gliomas. (A)
Shown are GL261 tumor volumes normalized to 100% on the first day of
treatment with CpG-1826 or controls (t = 0 days), mean ± SE, for
n mice per group: vehicle and GpC controls,
n = 4; CpG-1826, n = 9
until day 6, n = 5 until day 9, n
= 4 until day 12. *, p < 0.05 for CpG-1826 versus controls on
day 6 (one-tailed t-test). Arrows: days of treatment with CpG-1826 or controls.
(B) qPCR analysis of tumor RNA samples isolated from tumors in (A) and collected
on treatment day 6 (vehicle, control GpC, and CpG-1826 treatment) or day 12
(CpG-1826 treatment). *p < 0.05, n = 4 mice
per group. (C) Tumor growth curves in individual mice showing variable responses
to CpG-1826 given on days 3, 6, and 9 (arrows). (D) Normalized tumor volumes on
CpG-1826 treatment day 12 for CpG-1826 low-responsive mice (“CpG-1826,
Low”) versus CpG-1826 high-responsive mice (“CpG-1826,
High”), from panel C, with significance by one-way ANOVA with
Tukey’s post-test comparing CpG-1826-treated to untreated mice, and
low-responsive versus high-responsive mice, as indicated.