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. Author manuscript; available in PMC: 2017 Mar 1.
Published in final edited form as: J Immunol. 2016 Jan 25;196(5):2167–2180. doi: 10.4049/jimmunol.1501853

Figure 4. Particulate β-glucan induces respiratory burst and apoptosis in PMN-MDSC in a dectin-1 dependent manner.

Figure 4

(A) Representative dot plots showing the frequency of annexin V+ cells gated on Gr-1highCD11b+ PMN-MDSC in splenocytes of LLC-bearing mice cultured with media only or with particulate β-glucan (100 µg/ml) for indicated time. Summarized data are also shown (n=8). (B) Frequencies of annexinV+ cells gated on Gr-1highCD11b+ PMN-MDSC from WT or dectin-1 KO LLC-bearing mice cultured for 18 hours with or without particulate β-glucan (100 µg/ml) (n=2). (C) Respiratory burst in PMN-MDSC sorted from spleens of LLC-bearing WT or dectin-1 KO mice stimulated with particulate β-glucan (100 µg/ml) for indicated time. Reduction of dihydrorhodamine 123 (DHR) to fluorescent rhodamine 123 (RHO) was assessed by flow cytometry. Data is representative of 6 independent experiments (WT) and two independent experiments (KO). (D) Western blot analysis of p-STAT3, p-Zap/Syk, p-Akt, p-SAPK/JNK, p-Erk1/2, p-p38, STAT3 and β-actin in PMN-MDSC sorted from the spleens of LLC-bearing mice and treated with particulate β-glucan (100 µg/ml) for 0, 15, 30 and 60 minutes. Results are representative of at least three independent experiments. *p<0.05, **p<0.01, ***p<0.001.