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. Author manuscript; available in PMC: 2017 Mar 1.
Published in final edited form as: J Immunol. 2016 Jan 25;196(5):2377–2387. doi: 10.4049/jimmunol.1500770

Fig 2. Increased expression of eotaxin receptor CCR3 on lung endothelial cells in murine asthma model.

Fig 2

CCR3 expression was analyzed by immunohistochemistry on paraffin embedded lung sections and flow cytometry of lung single cell suspensions. [A] Naïve mouse lung endothelium showed expression of CCR3 at low levels. [B] Allergen exposed mouse lung had segments of the endothelium expressing CCR3. [C] Flow cytometric gating of lung endothelial cells (CD31+CD45-). [D & E] Analysis of CCR3 expression on the endothelial cells. [F] Open bar naïve mouse lung; closed bar OVA sensitized and day 6 ova challenged lung (n=3). Scale bar = 200 μm