Fig 4. Eotaxin Expression by Proangiogenic Progenitors is T_H2-Dependent.

[A] T_H2 response was induced in mice by sensitizing animals with OVA-aluminum hydroxide (OVA-alum) followed by OVA challenge. Proangiogenic hematopoietic progenitors (PACs) were isolated and expression of eotaxins was analyzed. Cytospins of proangiogenic progenitors were made, and immunofluoresence quantification for eotaxin was performed. Quantification of eotaxin content/cell. Box plot graphs of 6 mice in each group are shown. Eotaxin-2 was not quantified in this experiment [B] Proangiogenic hematopoietic progenitors were isolated from naÏve mice and ex vivo stimulated with T_H2-regulators IL-25 or IL-33. Eotaxin was quantified on cytospins as described under [A] or Methods section. Both eotaxin 1&2 were upregulated by IL-25 or IL-33 in a dose dependent-manner. Box plot graphs of 4 mice/group are shown.