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. Author manuscript; available in PMC: 2017 Mar 1.
Published in final edited form as: J Immunol. 2016 Jan 25;196(5):2377–2387. doi: 10.4049/jimmunol.1500770

Fig 5. Eotaxin-1/2 deficient bone marrow transplantation into wildtype mice reduced pathological angiogenesis and eosinophilic airway inflammation.

Fig 5

[A] Wildtype or eotaxin-1/2 deficient mice were sensitized and challenged with OVA allergen. Twenty-four hours after the final allergen exposure, animals were euthanized for analysis. [B] Lung angiogenesis was quantified on paraffin embedded tissue sections stained for endothelial cell marker von Willebrand Factor (vWF). OVA/OVA wildtype mice had a higher microvessel density compared to OVA/OVA eotaxin-1/2 deficient mice. [C] Airway inflammation was analyzed by staining lung sections for hematoxylin & eosin. Eosinophils in the bronchoalveolar lavage (BAL) were quantified by differential staining of BAL cytospins with Diff-Quick. Airway inflammation was blunted in OVA/OVA eotaxin-1/2 deficient compared to OVA/OVA wildtype mice. Black arrows indicate inflammatory cells. [D] Bone marrow mononuclear cells isolated from wildtype or eotaxin-1/2 deficient mice were engrafted into sublethally irradiated wildtype mice. Four weeks after the bone marrow transplantation animals were sensitized and challenged with OVA allergen or PBS. Twenty-four hours after the final allergen exposure, animals were euthanized for analysis. [E] Lung angiogenesis was quantified on paraffin embedded tissue sections stained for endothelial cell marker von Willebrand Factor (vWF). Compared to recipients of wildtype bone marrow, animals receiving eotaxin-1/2 deficient bone marrow exhibited significantly decreased microvessel density in the OVA/OVA group. [F] Airway inflammation was analyzed by staining lung sections for hematoxylin & eosin. Eosinophils in the bronchoalveolar lavage (BAL) were quantified by differential staining of BAL cytospins with Diff-Quick. Airway inflammation was significantly reduced in OVA/OVA wildtype mice reconstituted with eotaxin-1/2 deficient bone marrow. Black arrows indicate inflammatory cells. [G – H] Recipients of wildtype or eotaxin-1/2 deficient bone marrow in the PBS/PBS groups showed no increase in lung microvessel density or airway inflammation. Mean ± SE values of 4 mice/group are shown. Low power (200×) and high power (400×) images are shown. Scale bar in each image represents 100 μm.