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. Author manuscript; available in PMC: 2017 Mar 1.
Published in final edited form as: J Immunol. 2016 Feb 1;196(5):2181–2194. doi: 10.4049/jimmunol.1500303

FIGURE 9.

FIGURE 9

CGRP administered intradermally can modulate the immune response in vivo. Groups of BALB/c mice were injected intradermally with medium alone or CGRP. They were then immunized by application of DNFB at sites of injection. Three days later draining lymph nodes were harvested, a single-cell suspension of CD4+ lymphocytes were stimulated in culture with anti-CD3 and anti-CD28 monoclonal antibodies. After 72 hours, supernatants were harvested and cytokine content assessed by ELISA. Production of IL-17A and IL-4 were significantly increased in lymphocytes obtained from mice immunized at CGRP-treated sites compared to those at medium-treated sites while production of IFNγ and IL-22 was significantly decreased. n=10 mice per group for all groups except IL-17A, for which n=15 mice. *p < 0.05, **p < 0.01, ***p < 0.001.