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. Author manuscript; available in PMC: 2017 Feb 1.
Published in final edited form as: Free Radic Biol Med. 2015 Oct 22;91:143–153. doi: 10.1016/j.freeradbiomed.2015.10.408

Figure 5. Effect of 4-HNE on glycolysis in the presence of thrombin.

Figure 5

(A) Platelets were incubated with 4-HNE (HNE) (0–30 µM) for 1h after which basal ECAR was measured, followed by sequential injections of thrombin (0.5 U/ml) and oligomycin (O) (1 µg/ml). Indices of glycolytic function (B) thrombin-linked (TL) (thrombin ECAR) and (C) oligomycin sensitive (OS) (oligomycin ECAR – thrombin ECAR) were calculated. Data expressed as mean±SEM from one representative donor, n = 5–6 replicates. **p<0.01, different from thrombin.