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. Author manuscript; available in PMC: 2017 Feb 1.
Published in final edited form as: Free Radic Biol Med. 2015 Oct 22;91:143–153. doi: 10.1016/j.freeradbiomed.2015.10.408

Figure 8. Modification of platelet proteins by A-HNE.

Figure 8

Platelets were incubated with either media or AHNE (30 µM) for 1h, after which Click Chemistry was performed and the biotinylated proteins were pulled down with NeutrAvidin beads. The lysates were separated using SDS-PAGE gel and (B) western blot for streptavidin and (B) western blot for actin, citrate synthase and ATP synthase subunit α were performed. Data from an individual platelet donor.