Table III.
TCRB CDR3 sequences and gene use by polyclonal HSV-2-reactive CD8 T-cells subpopulations sorted with tetramers of HLA B*1502 and HSV UL48 160-168 or VZV ORF10 164-172.
| TCRBV | TCRBJ | CDR3 | bulk HSV-2- reactive |
HSV tetramer (+) VZV tetramer (+) |
HSV tetramer (+) VZV tetramer (−) |
|---|---|---|---|---|---|
| read % (rank) a | |||||
| 27-01*01 | 01-05*01 | CASSLSEDSNQPQHF | 9.41 (1st) | 1.06 (5th) | 0.99 (8th) |
| 06-05*01 | 02-07*01 | CASSRDLLEQYF | 4.21 (2nd) | 0.26 (12th) | 0.15 (18th) |
| 06 | 01-06*01 | CASSHDRGGHNSPLHF | 4.15 (3rd) | 3.58 (3rd) | 30.74 (2nd) |
| 02-01*01 | 02-01*01 | CASSMSSYNEQFF | 3.45 (4th) | 0.35 (11th) | 0.25 (12th) |
| 06-01*01 | 01-01*01 | CASGRQDENTEAFF | 2.31 (5th) | 0.07 (112th) | not found |
| 27-01*01 | 01-06*01 | CASKIGTGASPLHF | 2.31 (7th) | 0.23 (13th) | 45.48 (1st) |
| 12 | 01-01*01 | CASNRQGARNTEAFF | 0.79 (21st) | 68.33 (1st) | 0.71 (8th) |
| 14-01*01 | 02-01*01 | CASSQYRVDEQFF | 0.33 (70th) | 0.24 (13th) | 5.31 (3rd) |
| 14-01*01 | 02-05*01 | CASSQLRRETQYF | 0.12 (137th) | 1.02 (6th) | 2.79 (4th) |
| 06-05*01 | 01-01*01 | CASSYGRGRRNTEAFF | 0.15 (119th) | 5.83 (2nd) | 1.66 (5th) |
| 01-01*01 | 02-07*01 | CTSSHCDTGTTSVYEQYF | 0.08 (184th) | 1.67 (4th) | 0.22 (14th) |
| reads | 4,614,956 | 27,407 | 97,518 | ||
| productive reads (%) |
85.01 | 89.5 | 94.5 | ||
| unique productive reads |
3,039 | 98 | 173 | ||
| clonalityb | 0.42 | 0.72 | 0.69 | ||
a
Percent of CDR3 reads and rank amongst total reads. Top five CDR3 sequences bolded.
b
Per Adaptive algorithm. A monoclonal population is 1.0. No enrichment for any CDR3 sequence is 0.