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. 2015 Nov 7;100:2693–2707. doi: 10.1007/s00253-015-7098-8

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© The Author(s) 2015

Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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Fig. 1 — Batch bioreactor production of the alpha-amylase in S. cerevisiae INVSc1-pYES2-Amy1. a Time-course accumulation of the recombinant alpha-amylase in the medium and the cellular fraction, b carbon source and inducer concentration and biomass accumulation in a representative S. cerevisiae INVSc1-pYES2-Amy1 batch bioreactor culture. Y axis: closed circles—alpha-amylase activity in the medium; open circles—alpha-amylase activity in the cell; closed diamond—biomass growth in OD600 units; closed triangle—raffinose concentration; closed square—galactose concentration. X axis: time of culturing in h. Error bars indicate ±SD. c SDS–PAGE electrophoretic separation of the total intracellular and extracellular protein fractions of the INVSc1 and the INVSc1-pYES2-Amy1 strains. Proteins contained within the culture medium (Med), intracellular proteins (Cell), parental strain INVSc1 (WT), the recombinant strain (Rec—INVSc1-pYES2-Amy1). Asterisk (approx. 53 kDa) indicates the protein band, corresponding to the molecular weight of the recombinant amylase, expressed in the recombinant strain. PMWM protein molecular weight marker (PageRuler Prestained Protein Ladder, LifeTechnologies)