Table 2.

Kinetic parameters describing production of the recombinant alpha-amylase in S. cerevisiae INVSc-pYES2-Amy1 batch bioreactor cultures

	% of total proteins [[mg/mL AMYa]/ [mg/mL total proteins]] [%]		% of AMY secreted [medium mg/ total mg] [%]	Volumetric productivity Medium [AU/[L*h]] ± SD
time h	medium	cell
10	-	-	-	2.74 ± 0.14
22	72.58	0.017	99.98	2.53 ± 0.004
26	30.12	0.023	99.92	1.38 ± 0.002
46	7.76	0.024	99.69	0.68 ± 0.039
50	7.17	0.049	99.32	0.59 ± 0.02
70	6.99	0.019	99.72	0.54 ± 0.03

% of total proteins refers to the percentage of the alpha-amylase contained within the total amount of proteins, measured in the medium and in the cellular fractions. % of AMY secreted refers to the percentage of the alpha-amylase secreted. Volumetric productivity Medium refers to the volumetric productivity of the alpha-amylase, measured in the medium fraction

^amg/mL AMY was calculated based on the purified alpha-amylase activity. The enzyme was purified through affinity chromatography, to apparent homogeneity. 1 mg of the purified alpha-amylase contained 1.175 AU