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In vivo selection strategy for threonine aldolase activity based on the cleavage of β-hydroxy-α-amino acids. The threonine aldolase gene Cc-lTA is expressed from pMG-Ptet in the glycine auxotroph using the tetracycline-inducible Ptet system, which provides regulation of the stringency of the selection system by varying the inducer concentration (Giger et al. 2012)
