Skip to main content
. 2016 Feb 2;8:271–284. doi: 10.1016/j.redox.2016.02.001

Fig. 3.

Fig. 3.

Pi requirement for spontaneous extracellular catabolism of purine ribonucleosides. (A) Cytosol only without KH2PO4 (control) or cytosol only with added KH2PO4. (B–D) Cytosol plus an added purine ribonucleoside and NADP+ without KH2PO4 (control) or with added KH2PO4. Presence of a purine ribonucleoside carbon chain fuel source and NADP+ added to individual experiments are indicated above each panel (B–D). NADPH generation was determined by measurement of GSH produced from 3mM GSSG added to all experiments (A–D). X-axes show control experiments (no Pi added) and experiments supplemented with Pi. Y-axes show on the left of panel μM GSH generated and on the right of the panel μM Pi added or consumed (coded bars). 75 ng/μl cytosolic protein added to all experiments (A–D). Incubations were for 1200 min at pH 6.9 at 37°. Data are the mean (±SEM) of three separately performed experiments.