Table 2.

DNA extraction with the warm proteinase K digestion and cold 4 M GTC methods.

Method	Tissues	Tissue amount (mg)	DNA amount (µg)	Yields of DNA (µg/mg)	dG (µg/l)	8-oxodG (µg/l)	8-oxodG/dG (10−5)
The warm proteinase K digestion method	Normal A	93	398	4.28	13,200	0.6	4.5
Normal B	50	269.2	5.38	263	n/aa	n/aa
Tumor 1	70	313	4.47	9620	3	3.1
Tumor 2	60	280	4.67	14,830	0.7	4.7
The cold 4 M GTC method	Normal A	63	477	7.57	18,000	0.4	2.2
Normal B	55	279	5.07	4620	n/a	n/a
Tumor 1	125	n/aa	n/a	12,500	0.2	1.6
Tumor 2	60	11.9	0.19	12,150	0.4	3.3
Positive control	Calf thymus DNA	–	100		9800	7.6	77.5
Calf thymus DNA	–	60		8250	5.9	71.5

^anot detected