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. 2016 Jan 12;8(1):34–47. doi: 10.18632/aging.100870

Figure 4. Sirt6KO BMDCs show impaired maturation and CCR7 expression in response to TLR ligands.

Figure 4

(A-C) WT and Sirt6KO BMDCs were harvested at day 7 and stimulated with or without LPS or with CpG for 24 h. Thereafter, cells were harvested, washed, and MHCII, CD86, CD80, CD40 and CCR7 expression on CD11c+ cells was analyzed by flow cytometry. (A, B) One representative experiment out of ten (A) or out of four (B) is presented (n=4-21 for each genotype). (C) CCR7 mean fluorescence intensity (MFI) was normalized to that of WT BMDCs. Results are means ± SEM of four separate experiments, n=11 for each genotype; ***: p<0.001. (D) WT and Sirt6KO BMDCs were harvested at day 7 and stimulated for 24 h with LPS. Thereafter, cell migration to CCL21 was evaluated. The percentage of Sirt6KO BMDCs that had migrated was normalized to that of WT BMDCs. Results are means ± SEM of three separate experiments, n=4 for each genotype; *: p<0.05.