Table 1.

Oligonucleotide Primers and TaqMan Hybridization Probes Used in PCR Assays for Identifying Escherichia coli Heat-Labile (LT1) and Heat-Stable (ST1) Genes Indicative of Enterotoxigenic E. coli^a

Geneb,c	Sequence	Product Size
eltB		73 bp
eltB-205	5′ TAA GAG CGG CGC AAC ATT T 3′
eltB-277	5′ TTC AAT GGC TTT TTT TTG GGA 3′
eltB probe	5′ TTG ACT GCC CGG GAC TTC GAC CT 3′
estA1		151 bp
estA1-353	5′ AGT CAA CTG AAT CAC TTG ACT CTT CA 3′
estA1-503	5′ CCA GCA CAG GCA GGA TTA CA 3′
estA1 probe	5′ AAT CAG AAA ATA TGA ACA ACA CAT TTT ACT GCT GTG AA 3′
estA2,3		139 bp
estA2,3-197	5′ CCT TTC GCT CAG GAT GCT AAA C 3′
estA2,3-335	5′ ACA ATT CAC AGC AGT AAT TGC TAC TAT TC 3′
estA2 probe	5′ CGA TTC TAG TGT AAT TTT TTC TTT TGA AGA CCC TGC T 3′
estA3 probe	5′ AGT AGA GTC TTC AAA AGA AAA AAT CAC ACT AGA ATC A 3′

Abbreviations: bp, base pairs; PCR, polymerase chain reaction.

^a The SYBR Green and TaqMan primers and probes were developed by Minnesota Department of Health (unpublished data).

^b DNA oligonucleotide primers were synthesized by Integrated DNA Technologies, Inc. (Coralville, IA).

^c Hybridization probes (5′ FAM/TAMRA-Q 3′) were synthesized by Operon Biotechnologies, Inc. (Huntsville, AL).