FIG. 5.
Comparison of viral kinetics in hu-PBMC-NSG mice infected with two different tropic HIV-1 strains, HIV-1DH12 and HIV-1NL4-3. (A) Human CD4+ T cell depletion (line) and HIV-1 p24 Ag detection (square) in hu-PBMC-NSG mice (n = 5) infected with 1 × 103 IU of HIV-1DH12 4 weeks after intraperitoneal injection of human PBMCs. (B) Human CD4+ T cell depletion (line) and HIV-1 p24 Ag detection (square) in hu-PBMC-NSG mice (n = 5) infected with 1 × 103 IU of HIV-1NL4-3 4 weeks after intraperitoneal injection of human PBMCs. Mice were bled at the indicated time points after infection with HIV-1DH12 or HIV-1NL4-3; PBMCs were isolated for quantification of cell subsets by flow cytometry, and HIV-1 p24 levels in blood were analyzed by ELISA. Results are expressed as means ± standard deviation (error bars).