FIG. 3.
Generation of cultured TCM cells latently infected with HIV-1 NLENG1-IRES and reactivation of latent HIV-1. (A) Plasmid used for the generation of latently infected cells with an EGFP reporter. (B) Protocol for the generation of cultured TCM cells latently infected with HIV-1 NLENG1-IRES. (C) Cells were cultured and infected with HIV-1 NLENG1-IRES. On day 14, cultures were treated with IL-2 alone or IL-2+αCD3/αCD28 or IL-2+PHA. EGFP expression was measured using flow cytometry on day 16. Representative dot plots are shown for IL-2 and IL-2+αCD3/αCD28-treated cultures.