Table 1.
Examples of gene annotations from a single control Acropora aspera virome (generated from five coral fragments), including those from Herpesviridae, the Nucleocytoplasmic Large DNA Viruses (NCLDVs), and Retroviridae.
| Viral family annotation | ENA Accession No. | Contig coverage | nt Size | E-Value | aa % Identity | Viral genome similarity | Gene annotation |
|---|---|---|---|---|---|---|---|
| Herpesviridae | LT009377 | 6.43 | 511 | 2e – 44 | 52% | Elephant endotheliotropic herpesvirus 6 | Uracil DNA glycosylase |
| Herpesviridae | LT009378 | 1.18 | 497 | 2e – 17 | 34% | Gorilla lymphocryptovirus 2 | DNA polymerase |
| NCLDV/Mega | LT009379 | 2.5 | 635 | 2e – 11 | 28% | Pyramimonas orientalis virus | MutS protein |
| NCLDV/Mega | LT009380 | 1.34 | 321 | 4e – 53 | 64% | Lausannevirus | Eukaryotic peptide chain release factor subunit 1 |
| NCLDV/Mega | LT009381 | 2 | 907 | 2e – 08 | 30% | Acanthamoeba polyphaga mimivirus | Putative serine/threonine-protein kinase/receptor |
| NCLDV/Mega | LT009382 | 5.1 | 896 | 1e – 34 | 36% | Megavirus chilensis | UDP-N-acetylglucosamine 2-epimerase |
| NCLDV/Mega | LT009383 | 1.94 | 736 | 2e – 10 | 49% | Cafeteria roenbergensis virus BV-PW1 | Superfamily II helicase/eIF-4AIII |
| NCLDV/Mega | LT009384 | 2.45 | 751 | 2e – 22 | 45% | Cafeteria roenbergensis virus BV-PW1 | Putative photolyase |
| NCLDV/Mega | LT009385 | 1.58 | 259 | 7e – 21 | 67% | Cafeteria roenbergensis virus BV-PW1 | Putative DnaK/Hsp70 |
| NCLDV/Phyco | LT009386 | 2.37 | 1209 | 3e – 13 | 31% | Bathycoccus sp. RCC1105 virus | UDP-N-acetylglucosamine O-acyltransferase |
| NCLDV/Phyco | LT009387 | 5.75 | 720 | 7e – 09 | 47% | Paramecium bursaria Chlorella virus | ATP-dependent helicase |
| NCLDV/Pox | LT009388 | 1.76 | 880 | 3e – 09 | 31% | Pigeonpox virus | Hypothetical protein fep_013 |
| Retroviridae | LT009389 | 1.1 | 666 | 3e – 07 | 26% | Walleye epidermal hyperplasia virus 1 | RT_ZFREV_like reverse transcriptases |
| Retroviridae | LT009390 | 2.38 | 474 | 6e – 19 | 40% | Walleye epidermal hyperplasia virus 2 | Polymerase protein |
| Retroviridae | LT009391 | 4.7 | 856 | 5e – 15 | 32% | Reticuloendotheliosis virus | gag protein |
| Retroviridae | LT009392 | 3.2 | 474 | 6e – 13 | 46% | Reticuloendotheliosis virus | Envelope glycoprotein |
| Retroviridae | LT009393 | 4.86 | 461 | 2e – 14 | 48% | Reticuloendotheliosis virus | Protease |
| Retroviridae | LT009394 | 2.18 | 466 | 2e – 11 | 28% | Simian foamy virus | Integrase core domain |
Annotations are based on tBLASTx to the NCBI viral database (e-value threshold of <10–7). Contig sequences can be obtained through the European Nucleotide Archive (ENA).