FIG. 3.

Viable Rex1 promoter reporter responds with stress treatment in a dose-dependent manner in flow cytometry assay. mESCs expressing Rex1 promoter reporter were cultured in 12-well plates till 30% confluence. Cells were then treated with 200–300 mM of sorbitol in 25 mM increments for 3 days. After trypsinization, cell suspensions were assayed by microplate reader. Histograms shown in this study are from three independent experiments and (a) indicates significant decrease in Rex1-RFP fluorescence at all sorbitol doses from 200 to 300 mM as determined by ANOVA followed by Dunnett two-sided post hoc test, n = 3, P < 0.05.